Cytotoxicity test of apple cider vinegar as a root canal irrigant against fibroblast cells

Background: Apple cider vinegar potentially can be used as an alternative to irrigation solutions because of its antibacterial compounds that inhibit Enterococcus faecalis, a pioneer bacteria cause root canal treatment failure. One the ideal solution requirements is it isn’t toxic oral cavity tissues, so it’s necessary run cytotoxicity test on apple solution. Cytotoxicity initial part evaluation dental material before by humans. was performed fibroblast cells contact with fibroblast, which are main in periodontal ligament around apical. The purpose this study analyze vitro effect ACV cells. Method: concentrations 0.31%, 0.63%, 1.25%, 2.5%, and 5% tested using MTS assay method. Result: results showed there agent against fibroblasts cell. percentage cell viability less than 70%. Conclusion: There irrigant